Sticky ends from different BsmI sites may not be compatible. SwaI is typically used at 25☌, but is 50% active at 37☌. Prolonged incubation with NdeI may lead to removal of additional nucleotides.Įfficient cleavage requires at least two copies of the PluTI recognition sequence.Įfficient cleavage requires at least two copies of the NarI recognition sequence. Sticky ends from different BstAPI sites may not be compatible. G C A N N N N N T G C C G T N N N N N A C G Sticky ends from different PfoI sites may not be compatible.
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